| Autor: |
Waki, Shoko, Yoshitake, Kazutoshi, Iwasaki, Ryohei, Ueda, Hiroshi |
| Zdroj: |
Nucleic Acids Symposium Series; November 2007, Vol. 51 Issue: 1 p121-122, 2p |
| Abstrakt: |
While there are many hybridization-based DNA sensors, few of them can detect native double-stranded DNA, which is most commonly found in physiological conditions. Here we made novel fluorosensor proteins comprised of a pair of two zinc fingers tethered with an N-terminal dimerization motif and a C-terminal yellow fluorescent protein fragment (split eYFP) to detect specific DNA sequence in a living bacteria. When E. coli Top10 cells harboring the plasmid encoding the fusion proteins and a test plasmid encoding target DNA sequence were induced for the protein expression, significant increase in fluorescence was observed, compared with the strain harboring a test plasmid without target DNA sequence. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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