| Autor: |
Bodén, Elisabeth, Evander, Magnus, Wadell, Göran, Bjersing, Lars, von Schoultz, Bo, Rylander, Eva |
| Zdroj: |
Acta Obstetricia et Gynecologica Scandinavica; 1990, Vol. 69 Issue: 2 p153-159, 7p |
| Abstrakt: |
Various methods presently available for the diagnosis of genital Human Papilloma Virus (HPV) were compared regarding their sensitivity in women referred for specific diagnosis and treatment because of atypical Pap smears or clinically suspected neoplasia.Colposcopic examination was performed in all cases. In addition to taking a second Pap smear, cell suspensions were made from 105 women and analysed by the Filter In Situ Hybridization (FISH) technique and tested for HPV 6+11 and HPV 16+18+31. The FISH technique was also used for the possible detection of HPV-DNA in a reference material comprising 119 apparently healthy women with normal Pap smears.Colposcopically directed cervical biopsies in altogether 196 specimens were obtained from 155 women for histopathological examination and also for the detection of HPV-DNA by the Southern blot hybridization technique. These specimens were tested for the presence of HPV 6, 11, 16, 18, 31 and 33.Three per cent of the women with and 34% without cytological signs of HPV in Pap smears had cervical intraepithelial neoplasia (CIN) 111 according to histopathology. CIN III was present in 35% of biopsies with and 59% of biopsies without histological signs of HPV in the biopsies.Altogether 46% of the women were HPV-DNA positive. Of the women analysed by Southern blot, 39% were HPV-DNA positive. Of the samples analysed by FISH, 27% with atypical cells were HPV-DNA positive, compared with 11% of the samples from reference women with normal cytology.Of the women positive for HPV 16/18, colposcopy revealed HPV signs and or dysplasia in 95%.The presence of HPV in precancerous epithelium is not easily confirmed, either by cytology, colposcopy, or histopathology.By using Southern blot technique, it can be established that HPV-DNA (67%) is frequently present in CIN III lesions. The FISH technique appeared to be as sensitive as Southern blot concerning the possibility of detecting HPV-DNA in benign tissue and neoplasia of mild to moderate degree. |
| Databáze: |
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