Generation of mutants of a strain producing the new antibiotic batumin by means of transposon mutagenesis

Autor:	Smirnov, V. V., Churkina, L. N., Kravets, A. N., Perepnikhatka, V. I.
Zdroj:	Applied Biochemistry and Microbiology; May 2000, Vol. 36 Issue: 3 p262-265, 4p
Abstrakt:	Various plasmids carrying transposon Tn5 were used to generate insertion mutants synthesizing batumin, a unique antibiotic with a selective antistaphylococcal effect. One of the plasmids used provided a sufficient yield of the clones in question. An analysis of over 7000 clones allowed us to select the mutant clones with increased and decreased levels of batumin synthesis and the mutants that lost the ability to synthesize this antibiotic.
Databáze:	Supplemental Index
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