Production of antibodies to roridin and their applications

Autor: Burkin, A. A., Kononenko, G. P., Zoryan, V. G., Soboleva, N. A., Zotova, E. V.
Zdroj: Applied Biochemistry and Microbiology; July 2000, Vol. 36 Issue: 4 p368-371, 4p
Abstrakt: Polyclonal rabbit antibodies against a conjugate synthesized through condensing BSA and disubstituted roridin A hemisuccinate allowed roridin A to be determined in solutions at a sensitivity of 0.2 ng/ml. The cross-reactivity of structural analogues—roridin A, verrucarin A, and verrucarol—amounted to 100, 2.5, and 0.03%, respectively. The data showed that these antibodies determine roridin A in an indirect heterogeneous enzyme immunoassay in cereal straw samples at a sensitivity of 20 μg/kg.
Databáze: Supplemental Index