| Autor: |
Rus’, O. B., Puchkaev, A. V., Ivanov, A. I., Metelitsa, D. I. |
| Zdroj: |
Applied Biochemistry and Microbiology; January 2000, Vol. 36 Issue: 1 p36-45, 10p |
| Abstrakt: |
Difference spectrophotometry and fluorescence quenching of human and bovine serum albumins were used to determine their association constants(Ka) with hemin in buffered physiological saline (pH 7.4) supplemented with 2% dimethylsulfoxide or in 40% aqueous dimethylformamide (pH 7.4).Kavalues depended on the medium, the extent of albumin delipidation, and on the method of determination. The formation of hemin complexes witho-phenylenediamine, tetramethylbenzidine, gallic acid, its polydisulfide, and two substituted di-tert-butyl pyrocatechols was studied by difference spectrophotometry in the same media;Kavalues for the complexes were calculated and compared to each other. The formation of complexes of these aromatic ligands with albumins was studied fluorometrically;Kavalues were of order of ∼105M−1and decreased with the ligand hydrophobicity. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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