| Autor: |
MCKELVIE, J, FOSTER, A.P, HAMBLIN, A.S, CUNNINGHAM, F.M |
| Zdroj: |
Research in Veterinary Science; April 2001, Vol. 70 Issue: 2 p115-122, 8p |
| Abstrakt: |
Intradermal injection of a Culicoidesantigen extract (CAgX) induces T lymphocyte and eosinophil accumulation in the skin of horses with sweet itch. Blood mononuclear (BMN) cells from normal ponies proliferate when stimulated by mitogen (phytohaemagglutinin, PHA) or antigen (tetanus toxoid, TT) and, as shown here, release soluble factor(s) that induce eosinophil adherence. CAgX also caused concentration dependent proliferation ofBMNcells from sweet itch and normal ponies [stimulation index: 29 (13) and 17 (7) for BMNcells from sweet itch and normal ponies, respectively during the active phase of disease; 4 μg protein ml−1CAgX; 168 h]. A heat labile factor(s) which caused eosinophil adherence was also released [sweet itch ponies: 6.0 (1.6) per cent adherence versus 1.3 (0.4) per cent; normal ponies: 6.6 (0.5) per cent adherence versus 0.9 (0.1) per cent for supernatants from CAgX (4 μg protein ml−1; 48 hours) stimulated versus unstimulated BMNcells, respectively]. These results suggest that soluble proteins released from T lymphocytes could affect eosinophil function in the lesional skin of sweet itch horses. |
| Databáze: |
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