| Autor: |
Sudge, S. S., Bastawde, K. B., Gokhale, D. V., Kalkote, U. R., Ravindranathan, T. |
| Zdroj: |
Applied Microbiology and Biotechnology; May 1998, Vol. 49 Issue: 5 p594-599, 6p |
| Abstrakt: |
Abstract: About 1000 bacterial colonies isolated from sea water were screened for their ability to convert dl-5-phenylhydantoin to d(−)N-carbamoylphenylglycine as a criterion for the determination of hydantoinase activity. The strain M-1, out of 11 hydantoinase-producing strains, exhibited the maximum ability to convert dl-5-phenylhydantoin to d(−)N-carbamoylphenylglycine. The strain M-1 appeared to be a halophilic Pseudomonas sp. according to morphological and physiological characteristics. Optimization of the growth parameters revealed that nutrient broth with 2% NaCl was the preferred medium for both biomass and enzyme production. d-Hydantoinase of strain M-1 was not found to be inducible by the addition of uracil, dihydrouracil, β-alanine etc. The optimum temperature for enzyme production was about 25 C and the organism showed a broad pH optimum (pH 6.5–9.0) for both biomass and hydantoinase production. The organism seems to have a strict requirement of NaCl for both growth and enzyme production. The optimum pH and temperature of enzyme activity were 9–9.5 and 30 C respectively. The biotransformation under the alkaline conditions allowed the conversion of 80 g l−1dl-5-phenylhydantoin to 82 g l−1d(−)N-carbamoylphenylglycine within 24 h with a molar yield of 93%. |
| Databáze: |
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