Autor: |
Wullenweber, M., Boot, R. |
Zdroj: |
Laboratory Animals; October 1994, Vol. 28 Issue: 4 p335-339, 5p |
Abstrakt: |
Thirty-nine guineapigs were examined for Bordetella bronchisepticainfection by culture and serology, using the enzyme-linked immunosorbent assay (ELISA)and the indirect immunofluorescence (IIF) test. Each serological method detected evidence of B. bronchisepticainfection in more guineapigs than did culture. IIF using an antigen prepared from a mouse isolate of B. bronchisepticadetected fewer infected guineapigs than when performed with antigens prepared from B. bronchisepticaisolates from a rat, a dog and a guineapig. ELISA and IIF detected a comparable incidence of infection.Cross-reactivity was investigated further by carrying out ELISAs using 5 antigens prepared from B. bronchisepticaisolates from different species and antiserum to these antigens raised in non-infected guineapigs. |
Databáze: |
Supplemental Index |
Externí odkaz: |
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