| Autor: |
Lioubin, M. N., Meier, M. D., Ginsberg, B. H. |
| Zdroj: |
Preparative Biochemistry & Biotechnology; October 1984, Vol. 14 Issue: 4 p303-311, 9p |
| Abstrakt: |
We have developed a rapid method for producing homogeneous mono-[12]A14 iodoinsulin with high specific activity and yield. After iodination by the lactoperoxidase method, the labeled peptides were applied to a C18 PorasilR prs-column, washed with aqueous buffer to eliminate the free [12]-iodide and placed "in-line" with a C-18 HPLC column; mono-[12]A14 iodoinsulin was then eluted isocratically with 29% aceto-nitrile in 16 minutes. The labeled hormone was extremely stable, and proved suitable for various biological studies. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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