Isolation of Thymosin α1 from Thymosin Fraction 5 by High Performance Liquid Chromatography

Autor: Gabriel, Thomas, Meienhofer, Johannes, Michalewsky, Joseph
Zdroj: Journal of Liquid Chromatography & Related Technologies; March 1983, Vol. 6 Issue: 4 p647-654, 8p
Abstrakt: An HPLC system for quickly isolating individual peptides from thymus extract is described. A series of reversed phase separations followed by ion-exchange HPLC produces pure peptides with minimal chromatographic time. A 2.4 gram column load of crude extract was carried through to the isolation of 4 mg of pure thymosin α1 within 6-8 hours of chromatography.
Databáze: Supplemental Index