| Autor: |
de Vries, J. X., Schmitz-Kummer, E., Siemon, D. |
| Zdroj: |
Journal of Liquid Chromatography & Related Technologies; June 1994, Vol. 17 Issue: 10 p2127-2145, 19p |
| Abstrakt: |
A method for the quantitation of the enantiomers of the non-steroidal anti-inflammatory drug (NSAID) ibuprofen (IB) in human plasma and urine was developed for pharmacokinetic studies of the individual optical antipodes. Plasma samples were acidified, extracted with organic solvents and analysed by HPLC using an α1-acid glycoprotein column and UV detection; elution was performed with a phosphate buffer and isopropanol gradient; RS-flurbiprofen (FL) was used as internal standard. Calibration curves were linear in the range 0.25-25 μg/ml of each IB-enantiomer. Enantiomers and internal standards were baseline separated. Precision and accuracy was ± 3-6%, the limit of detection 0.1 μg /ml, and the analytical recoveries of IB and FL 93.7 ± 5% and 94.5 ± 4% resp.; endogenous substances, IB metabolites and other drugs did not interfere with the assay. Urine samples were extracted and analysed as for plasma to assay free, and after alkaline hydrolysis, total IB-enantiomers. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
