| Autor: |
Gao, Guang-Ping, Engdahl, Ryan K., Wilson, James M. |
| Zdroj: |
Human Gene Therapy; January 1, 2000, Vol. 11 Issue: 1 p213-219, 7p |
| Abstrakt: |
Production of E1-deleted adenovirus vectors for gene therapy has been plagued by the emergence of replication-competent adenovirus. A number of investigators have minimized homologous sequences between the vector and transfected E1 DNA in an attempt to avoid replication-competent adenovirus. We describe a HeLabased cell line called GH329 that stably expresses the E1 locus from a promoter derived from the phosphoglycerate kinase gene. Overlap sequences with a standard E1-deleted vector that retains a full pIX transcriptional unit have been eliminated at the 5 end and minimized at the 3 end. The GH329 cell line plaques and produces E1-deleted adenovirus as well as 293 cells. Replication-competent virus has emerged after 5 passages of vector on 293 cells but was not detected after 20 passages on GH329 cells. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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