| Autor: |
Tomilin, N. V., Barenfeld, L. S. |
| Zdroj: |
International Journal of Radiation Biology; 1979, Vol. 35 Issue: 3 p253-263, 11p |
| Abstrakt: |
γ-Endonuclease Y, an enzyme that hydrolyses phosphodiester bonds at alkalistable lesions in γ-irradiated (N2, tris buffer) DNA, has been partially purified from Micrococcus luteus. The enzyme has a molecular weight of about 19 000, induces single-strand breaks with 3'OH-5'PO4 termini and contains endonuclease activity towards DNA treated with 7-bromomethylbenz(a)anthracene. γ-Endonuclease Y induces breaks in OsO4-treated poly(dA - dT) and apparently is specific towards γ-ray-induced base lesions of the t' type. The complete excision repair of γ-endonuclease Y substrate sites has been performed in vitro by γ-endonuclease Y, DNA polymerase and ligase. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
