| Autor: |
Bölöni, Erzsébet, Szabó, L. D. |
| Zdroj: |
International Journal of Radiation Biology; 1978, Vol. 34 Issue: 4 p329-336, 8p |
| Abstrakt: |
The effect of 60Co γ irradiation on the activity of valyl-tRNA synthetase isolated from chick embryo brain was studied. The enzyme activity exponentially decreased in the dose range 10-200 krad. The first step of the enzyme action, i.e. the amino acid activation, was found to be less sensitive to irradiation than the whole reaction, the formation of valyl-tRNA. 2-Mercapto ethanol and/or glycerol had a significant radioprotective effect. The lesion caused by radiation in the enzyme was also influenced by its concentration during exposure (dilution effect). According to gel-electrophoretic experiments, no chain rupture occurred in the enzyme molecule. Not even a change in Km was observed; however, the maximum velocity of the reaction was found to decrease with increasing radiation dose. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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