Microspectrophotometric Study of Histoenzymic Reactions in Rat Epidermis Subjected to 0·5 Mrads of Deuteron Radiation

Autor: Cabrini, R. L., Itoiz, M. E., Mayo, J., Smolko, E. E., Klein-Szanto, A. J. P.
Zdroj: International Journal of Radiation Biology; 1970, Vol. 18 Issue: 5 p415-421, 7p
Abstrakt: The epidermis of new-born rats was irradiated with a deuteron beam of 26·5 MeV. 500 krads were delivered to 4 mm diameter circular areas in the infraorbicular left side of the heads.Quantitative histochemical determinations of NADP diaphorase, glucose-6-phosphate dehydrogenase, lactic and succinic dehydrogenase were performed, using a microspectrophotometric scanning technique, from 2 to 48 hours after irradiation.There was no clear change with LDh and SDh. A clear increment was detected in both total content and mean concentration of NADP Diaph. and Gl-6-phDh. No evidence of a real enzyme decrease shortly after irradiation could be detected by our techniques. This could imply that no significant direct radiation effects occur to the enzyme molecules in vivo.
Databáze: Supplemental Index