| Autor: |
Ellenberger, Dennis L., Pieniazek, Danuta, Nkengasong, John, Luo, Chi-Cheng, Devare, Sushil, Maurice, Chantal, Janini, Mario, Ramos, Artur, Fridlund, Carol, Hu, Dale J., Coulibaly, Issa-Malick, Ekpini, Ehounou, Wiktor, Stefan Z., Greenberg, Alan E., Schochetman, Gerald, Rayfield, Mark A. |
| Zdroj: |
AIDS Research and Human Retroviruses; January 1, 1999, Vol. 15 Issue: 1 p3-9, 7p |
| Abstrakt: |
To better understand the molecular epidemiology of HIV genetic diversity in Abidjan, Ivory Coast, we performed a genetic analysis of 170 HIV-1-seropositive specimens representing newly diagnosed tuberculosis patients (n = 143) and women monitored in a mother-to-child transmission cohort study (n = 27). Preliminary screening with RFLP presumptively classified 162 (95.3%) of these as subtype A. The envelope region of 108 specimens was subtyped by sequence analysis: 102 (94.4%) were subtype A, 2 (1.9%) were subtype D, and 4 (3.7%) were subtype G. Subtyping gag and env regions of the genome suggested that five of the six nonsubtype A isolates exhibited a potentially mosaic structure. A comparative phylogenetic analysis of HIV-1 subtype A C2V3 from 27 Ivory Coast and 21 Ugandan sequences revealed a striking clustering among Ivory Coast variants, and an independent segregation from Ugandan subtype A. Despite independent clustering with other subtype A specimens, limited variability of the V3 loop apex was observed; the globally predominant V3 motif, GPGQ, represented 90.1% of the HIV-1 strains. This study demonstrates that clade A is the predominant HIV-1 subtype in HIV-seropositive individuals in Abidjan, Ivory Coast and that these strains are phylogenetically distinct from other subtype A strains observed in East Africa. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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