| Autor: |
Charan, R. D., Munro, M. H. G., O'Keefe, B. R., Sowder, R. C., II, McKee, T. C., Currens, M. J., Pannell, L. K., Boyd, M. R. |
| Zdroj: |
Journal of Natural Products; August 25, 2000, Vol. 63 Issue: 8 p1170-1174, 5p |
| Abstrakt: |
Aqueous extracts from the African plant Myrianthus holstii potently inhibited the infection of the T-lymphoblastoid cell line, CEM-SS, by human immunodeficiency virus-1RF (HIV-1RF). The active constituent, M. holstii lectin (MHL), was purified by LH-20 column chromatography and reversed phase HPLC. MHL, a 9284-Da cysteine-rich protein, was characterized by amino acid analysis, N-terminal sequencing, ESIMS, and matrix-assisted laser-desorption ionization−time-of-flight mass spectrometry. Pure MHL had anti-HIV activity, with an EC50 value of 150 nM. Delaying the addition of MHL for up to 8 h after initial exposure of CEM-SS cells to virus did not result in loss of the antiviral activity; however, if addition of the compound was delayed for 16 h or more, there was a marked decrease in the antiviral activity. MHL bound to a virus-free, soluble form of the viral envelope protein gp120 but did not inhibit the subsequent binding to a cell-free, soluble form of the cellular receptor CD4. |
| Databáze: |
Supplemental Index |
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