| Autor: |
Kedes, Laurence H., Cohn, Ronald H., Lowry, Jean C., Chang, Annie C.Y., Cohen, Stanley N. |
| Zdroj: |
Cell; November 1975, Vol. 6 Issue: 3 p359-369, 11p |
| Abstrakt: |
Sucrose gradient analysis of total sea urchin DNA cleaved with theEcoRI andHindIII restriction endonucleases and identification of histone coding gene sequences by hybridization with histone mRNA have elucidated the basic organization of the histone gene repeat unit. These data, plus results obtained by electrophoretic analysis of purified endonuclease-cleaved sea urchin histone DNA and hybridization with cRNA transcribed from the eucaryotic segment of constructed plasmid chimeras cloned in E. coli, show that the several DNA sequences coding for individual histone proteins are intermingled in a 7 kilobase (kb) repeat unit. Cleavage of total sea urchin DNA withEcoRI produces 2.2 and 4.8 kb fragments which are homologous with the two cloned fragments, and which are contained in a 7 kbHindIII fragment. Cleavage with both enzymes reveals that the 2.2 kbEcoRI fragment contains aHindIII site 0.15–0.2 kb from an end. RNA · DNA hybridization between chimeric plasmid DNA and purified individual mRNAs isolated from sea urchin embryo polyribosomes has been used to assign coding sequences to either the 2.2 or 4.8 kb region of the histone DNA repeat unit. A map of the histone genes is proposed. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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