| Autor: |
Brynda, E., Houska, M., Wikerstal, A., Pientka, Z., Dyr, J. E., Brandenburg, A. |
| Zdroj: |
Langmuir; May 2000, Vol. 16 Issue: 9 p4352-4357, 6p |
| Abstrakt: |
Films consisting of three cross-linked molecular layers of human serum albumin (HSA) or three HSA layers alternating with three heparin layers were immobilized on surfaces of grating coupler sensors and hydrophobized glass. Optical responses of the coated sensors to pH changes were observed. A decrease in the effective refractive index, ΔNeff, measured by a grating coupler was observed when the pH of buffers contacting the films was increased. The process was quite reversible, which indicated that the mass of the film remained constant when the buffers were repeatedly exchanged. Theoretical treatment of the grating coupler optics related the decrease in ΔNeff to expansion of the films. The interpretation of sensor measurements was confirmed by atomic force microscopy which revealed reversible changes in the thickness of HSA/heparin film induced by pH changes of the buffers. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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