Detection of Coexisting Bilayer Gel and Fluid Phases by Equilibrium Surface Pressure Analysis

Autor: Buboltz, J. T., Feigenson, G. W.
Zdroj: Langmuir; April 2000, Vol. 16 Issue: 8 p3606-3611, 6p
Abstrakt: A study of bilayer phase behavior is presented for the phospholipid mixture di12:0-PC/di16:0-PC, based on an analysis of equilibrium surface pressures which develop at the gas−water interface above suspensions of bilayer vesicles. At 22 ± 1 °C, the fluidus and solidus bilayer phase boundaries are determined to be 32 and 85 mol % di16:0-PC, respectively, consistent with literature values obtained through calorimetry. Evidence is presented which suggests that Teflon substrates are better suited than high-energy substrates for surface pressure measurements involving insoluble monolayers.
Databáze: Supplemental Index