| Autor: |
Burks, A.Wesley, Cockrell, Gael, Connaughton, Cathie, Karpas, Arthur, Helm, Ricki M. |
| Zdroj: |
The Journal of Allergy and Clinical Immunology; February 1995, Vol. 95 Issue: 2 p607-611, 5p |
| Abstrakt: |
The antigenic and allergenic structure of Ara hII, a major allergen of peanuts, was investigated with the use of four monoclonal antibodies obtained from BALB/c mice immunized with purified Ara hII. Our previous studies with monoclonal antibodies generated to peanut allergens showed this method to be useful for epitope mapping. When used as a solid phase in an ELISA, these monoclonal antibodies captured peanut antigen, which bound human IgE from patients with positive peanut challenge responses. The Ara hII monoclonal antibodies were found to be specific for peanut antigens when binding for other legumes was examined. In ELISA inhibition studies with the monoclonal antibodies, we identified two different antigenic sites on Ara hII. In similar studies with pooled human IgE serum from patients with positive challenge responses to peanuts, we identified two closely related IgE-binding epitopes. These characterized monoclonal antibodies to Ara hII will be useful for future studies to immunoaffinity purify the Ara hII allergen and to use in conjunction with recombinant technology for determining structure-function relationships. (J ALLERGYCLINIMMUNOL1995;95:607-11.) |
| Databáze: |
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