| Autor: |
Cairoli, E., Varangot, M., Signorelli, S., Batthyany, C., Bianchi, S., Beuzelin, M., Phillips, E., Musé, I., Roseto, A., Osinaga, E. |
| Zdroj: |
European Journal of Cancer; November 1995, Vol. 31 Issue: 6 pS265-S265, 1p |
| Abstrakt: |
The Tn antigen (GaINAc-O-Ser/Thr) is one of the most specific human carcinoma associated structures. In order to measure soluble Tnglycoproteins, we have developed an immuno-lectin-enzymatic assay (CA83.4), in which a monoclonal antibody (83D4) is bound to the solid phase to capture glycoproteins bearing Tn determinant. After addition of test sample, biotinylated isolectin B4 from Vicia villosaand avidinperoxydase are used as a detection system. CA83.4 values were significantly elevated in the serum of 36/l02 patients with cancer (35.3%), 0/50 of patients with non malignant diseases and in none of 97 healthy controls. CA83.4 was also elevated in 74/85 of pleural or ascitic serous effusions associated with cancer (87%) but not in the 24 serous effusions from patients without cancer. All samples from patients with haematologic malignancies showed very low or undetectable levels of antigen. This first report on soluble Tn-glycoproteins detection suggests that CAR8.4 assay could be a specific serological tumor marker. A large study is in progress to determine the true clinical value of the test. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
Full Text from ScienceDirect