| Autor: |
Bellon, L., Workman, C. T., Jarvis, T. C., Wincott, F. E. |
| Zdroj: |
Bioconjugate Chemistry; March 26, 1997, Vol. 8 Issue: 2 p204-212, 9p |
| Abstrakt: |
To improve the overall yield of ribozyme synthesis, a convergent approach, based on the post-synthetic formation of an amino linker between two half-ribozymes was investigated. Borane·pyridine-mediated reductive amination of 3-phosphoglycaldehyde-5-half-ribozymes with 5-aminohexyl-3-half-ribozymes generated the corresponding amino-linked ribozymes in yields >77% on different scales. The investigation of a variety of reducing agents is discussed together with a kinetic analysis of the selected coupling reaction. These post-synthetically ligated ribozymes exhibited slightly reduced in vitro catalytic activity and cell efficacy. |
| Databáze: |
Supplemental Index |
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