| Autor: |
Rippel, G., Bede, A., Szepesy, L. |
| Zdroj: |
Journal of Chromatography A; April 1995, Vol. 697 Issue: 1-2 p17-29, 13p |
| Abstrakt: |
In order to rationalize the selection of the phase system (the stationary phase and the mobile phase) applicable for the separation of some proteins, gradient measurements were carried out on three columns of different types using three salts as eluent constituents. The slope and intercept of the lnk-salt concentration relationship for proteins varied with the different phase systems. The hydrophobicity index (c^*) showed how the alteration of the type of phase system affects the selectivity of separation. Two-dimensional mapping along the parameters of the slope-intercept relationship revealed the extent of the difference among the phase systems. In addition, the effect of solutions of binary and ternary salt mixtures on the retention of proteins was investigated. Measurements were carried out according to a mixture design and the retention time was modelled with the predictive method of Jandera and by fitting a polynomial to the data according to the Sentinel method. The accuracy of the former method were dependent on the type of phase system. The fit of second-order polynomial gave very good results but a first-order model was also acceptable. |
| Databáze: |
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