| Autor: |
Kurkijarvi, R., Yegutkin, G.G., Gunson, B.K., Jalkanen, S., Salmi, M., Adams, D.H. |
| Zdroj: |
Gastroenterology; October 2000, Vol. 119 Issue: 4 p1096-1103, 8p |
| Abstrakt: |
Background & Aims: Vascular adhesion protein 1 (VAP-1) is an endothelial glycoprotein that supports adhesion of lymphocytes to hepatic endothelium and has sequence homology with semicarbazide-sensitive amine oxidases (SSAOs). We investigated whether soluble VAP-1 (sVAP-1) displays SSAO activity and thereby accounts for increased monoamine oxidase activity in the serum of patients with liver diseases. Methods: sVAP-1 concentration and SSAO activity were measured in peripheral, hepatic, and portal blood and in bile from patients with liver disease and in peripheral blood of control subjects, using enzyme-linked immunosorbent assay and enzymatic assays. Results: sVAP-J. concentration (mean [+/-SE], 143.67 [34.97-92.67] ng/mL) and SSAO activity (18.8 [12.0-24.6] nmol . mL^-^1 . h^-^1) were significantly increased in chronic liver diseases compared with healthy controls (87.1 [53.5-127] ng/mL [P < 0.001] and 10.7 [6.5-12.7] nmol . mL^-^1 . h^-^1 [P < 0.05]) but not in massive necrosis caused by paracetamol poisoning (109 [80.3-140] ng/mL and 8.9 [5.7-12.3] nmol . mL^-^1 . h^-^1). sVAP-1 correlated with serum transaminase and bilirubin but not with creatinine. In 5 paired samples, sVAP-1 concentration was higher in hepatic (median, 113 [range, 53-122]) than in portal vein (102 [42-109]; 2P <0.05), and was not detected in bile. There was a highly significant correlation between serum sVAP-1 and SSAO activity in normal subjects, patients with acute liver failure, and those with chronic liver disease (r = 0.895; P < 0.001). When serum was depleted of sVAP-J. by immunoaffinity chromatography, SSAO activity was eliminated. Conclusions: sVAP-1 levels are increased in chronic liver disease, and sVAP-1 is likely derived from the liver. Serum sVAP-1 displays SSAO activity and accounts for most of the monoamine oxidase activity in human serum. |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
