| Abstrakt: |
The analysis and synthesis of information on the field of use, sources, mechanism of action and properties of the enzyme lipase was performed in a review. It is established that for sectors of the economy in which lipase successfully used or is planned to be used is constantly expanding. Performed patent search of lipase modern producers has found among these bacteria, actinomyces, yeast and microscopic fungi. The features of the formation process of culture media and cultivation of lipase producers profound way. Detailed description of modern technologies lipases with varying degrees of treatment D2x, D3x, D10x is presented. It is established, that for obtaining of highly purified enzyme preparations of lipases fractionation using ion exchange and gel-filtration is used the most often. The latest alternative methods of treatment are described. Modern methods of immobilization of lipases are presented. It is shown that the physical adsorption immobilization methods are used, among chemical -- method of covalent binding of the enzyme with the carrier. The possibility of immobilization of enzymes on the surface of yeast cells was outlined. Methods of determining the target of the activity of lipase, which include alkalimetric titration and colorimetric method were established. Range of manufacturers of lipolytic enzymes, which are focused today outside the commonwealth of independent states was outlined. [ABSTRACT FROM AUTHOR] |
