Cofermentation of glucose and galactose by a newly isolated Saccharomyces cerevisiae strain in free and immobilized forms.

Autor: Wu, Fang-Chen, Wu, Jane-Yii, Liao, Yi-Jyun, Wang, Man-Ying, Shih, Ing-Lung
Předmět:
Zdroj: Journal of the Taiwan Institute of Chemical Engineers; Sep2014, Vol. 45 Issue 5, p2387-2394, 8p
Abstrakt: A newly isolated yeast strain from the soil of grape fruit farm was identified as Saccharomyces cerevisiae Wu-Y2 through molecular characterization. This strain, whether used in free or immobilized form, showed a good performance on co-fermentation of reagent grade mixed sugars comprising of glucose and galactose in production of bioethanol in batch and fed batch culture. The yield coefficient ( Y E/S ; 0.47 g/g) for ethanol from monosaccharide and yield efficiency (YE; 92.0%) after 48 h fermentation of mixed sugars shown by this wild-type S. cerevisiae Wu-Y2 were much higher than those by engineered S. cerevisiae (D452-2BT) strain (0.34 g/g, 67%) and engineered industrial distillers’ yeast (0.28 g/g and 55%). The immobilized S. cerevisiae Wu-Y2 cells exhibited a high degree of operational stability for several weeks without significant loss of viability or ethanologenicity in fed batch culture and it can tolerate up to 7–10% of ethanol for more than a month. This strain also showed good ability to utilize glucose and galactose present in red seaweed hydrolysate with high ethanol conversion efficiency; it showed significant potential to be an efficient bioethanol producer using seaweed as a feedstock. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index
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