| Autor: |
FIRA, Alexandru, CLAPA, Doina, SIMU, Manuela |
| Předmět: |
|
| Zdroj: |
Bulletin of the University of Agricultural Sciences & Veterinary Medicine Cluj-Napoca. Horticulture; 2014, Vol. 71 Issue 1, p29-37, 9p |
| Abstrakt: |
Our paper presents the results of the application of a micropropagation protocol developed at the Fruit Research Station Cluj, in Rubus fruticosus cultivars 'Čačanska bestrna', 'Chester Thornless', 'Loch Ness' and 'Navaho'. For the in vitro multiplication stage, Murashige & Skoog [MS) media supplemented with 0.3 and 0.5 mg/1 BAP and gelled with starch were used. Direct ex vitro rooting was carried out. The shoots regenerated in vitro in the multiplication stage were rooted ex vitro in two experimental treatments: float hydroculture and floating perlite beds. In cultivars 'Loch Ness' and 'Chester' 0.5 mg/1 BAP yielded good results regarding in vitro proliferation and multiplication rates and the regenerated shoots were vigorous, suitable for ex vitro rooting and acclimatization. In cultivars 'Navaho' and 'Čačanska Bestrna' the optimal BAP concentration was 0.3 mg/1, which ensured normal plantlet development. The experimental treatments with 3 and 4 microcuttings / vessel gave good results, whereas the ones with 1 and 2 microcuttings / vessel did not yield satisfactory results regarding growth and proliferation. Wheat starch was successfully used as a gelling agent in all the cultivars. In cultivar 'Čačanska bestrna' potato starch gave very good results regarding in vitro proliferation. In the direct ex vitro rooting experiments floating perlite gave good results in all the cultivars. In cultivar 'Čačanska bestrna' ex vitro rooting in float hydroculture gave relatively poor results, with rooting percentages around 60 %. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
|
