Large-Scale Survey of Mitochondrial D-Loop of the Red-Crowned Crane Grus japonensis in Hokkaido, Japan by Convenient Genotyping Method.

Autor: Yoshiaki MIURA, Akira SHIOMI, Junya SHIRAISHI, Kohei MAKITA, Mitsuhiko ASAKAWA, Takio KITAZAWA, Takeo HIRAGA, Yulia MOMOSE, Kunikazu MOMOSE, Hiroyuki MASATOMI, Hiroki TERAOKA
Předmět:
Zdroj: Journal of Veterinary Medical Science; Jan2013, Vol. 75 Issue 1, p43-47, 5p
Abstrakt: The article presents a study that developed a rapid and inexpensive method of extensive genotyping of D-loop haplotypes in Red-crowned Cranes in Hokkaido, Japan based on the amplification refractory mutation system (ARMS) polymerase chain reaction (PCR) assay. Particular focus is given to the nucleotide sequencing of the mitochondrial D-loop, ARMS PCR assay for D-loop typing, and crane sex identification process. It discusses the conventional sequencing of the D-loop.
Databáze: Supplemental Index