| Autor: |
Wada, Sanae, Mikuni, Tatsuo, Atsumi, Shogo, Mitsuhashi, Wataru, Narukawa, Junko, Kadono-Okuda, Keiko, Yamamoto, Kimiko, Mita, Kazuei, Noda, Hiroaki, Miyamoto, Kazuhisa, Kunimi, Yasuhisa |
| Předmět: |
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| Zdroj: |
Journal of Insect Biotechnology & Sericology; Feb2012, Vol. 81 Issue 1, p1-6, 6p |
| Abstrakt: |
Resistance to the entomopathogenic fungus Beauveria brongniartii (Sacc.) is controlled by a dominant gene in the silkworm, Bombyx mori (Linnaeus) (Lepidoptera: Bombycidae). We determined the linkage group by which the resistance gene links through linkage analysis with the linkage map of the silkworm based on single nucleo-tide polymorphisms (SNPs). Taking advantage of the lack of crossing over in females of the silkworm, back-crossed F1 progenie0s (BC1) made by crossing an F1 female (susceptibility-developed strain AkakoS x resistance-developed strain p50TR) and a male AkakoS were used. The resistant BC1 individuals were selected by screening of injection with conidial suspension of B. brongniartii. We selected SNP markers that were able to dis-tinguish the genotypes of AkakoS and p50TR on each of the 28 linkage groups and analyzed the genotypes of the resistant BC1 individuals on each linkage group with the SNP markers. There are two genotypes in BC1, homo (AkakoS) type and hetera (F1) type, but only hetero type BC1 individuals can be resistant to B. brongniartii on the linkage group to which the resistance gene links. All resistant BC1 individuals showed hetero type only at the 14th linkage group, suggesting that the resistance gene of the silkworm to B. brongniartii exists on the 14th linkage group. We designated this gene as "resistant to B. brongniartii' with the abbreviation Rbbr. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
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