Autor: |
Grosser, Robert J., Morris, Brian, Carson, David A., Davis-Hoover, Wendy J. |
Zdroj: |
Journal of Solid Waste Technology & Management; May2013, Vol. 39 Issue 2, p87-100, 14p, 4 Charts, 3 Graphs |
Abstrakt: |
Research on the conversion of conventional municipal solid waste landfills to landfill bioreactors and the installation of landfills as bioreactors is ongoing to determine their costs and benefits. The research described in this paper utilized different laboratory methodologies for enumerating microbial populations present in landfill leachate samples. Leachate samples were taken quarterly over a two year period from Outer Loop Landfill in Louisville, Kentucky, USA, a municipal solid waste landfill where three treatment processes are being compared at the field-scale: newly constructed and converted bioreactor treatments, and conventionally managed landfills as a control treatment. Leachate samples were processed using classical microbiological culture-dependent techniques as well as culture-independent molecular techniques to enumerate total Eubacteria, sulfate reducers, denitrifiers, and methanogens. Total community DNA was extracted and bacterial 16s rDNA was subsequently amplified and cloned. Results show that DNA molecular techniques were more variable and gave both higher and lower bacterial num-bers than culturing techniques. The concentrations of all microbial groups varied over time, with the most obvious variation found for the methanogens. [ABSTRACT FROM AUTHOR] |
Databáze: |
Supplemental Index |
Externí odkaz: |
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