Development of fluorescence quantitative PCR assay to detect gB gene of pseudorabies virus.

Autor: Shu-fen, ZHU, Rui-liang, ZHU, Cai-xia, QIAO, Zhi-qiang, GAO, Li-feng, ZHANG, He-xiao, ZHANG, Yia-qiong, WU, Hui-shan, WANG
Zdroj: Chinese Journal of Veterinary Science / Zhongguo Shouyi Xuebao; Oct2012, Vol. 32 Issue 10, p1413-1417, 5p
Abstrakt: The article presents information on a study regarding the development of fluorescence quantitative polymerase chain reaction (PCR) assay to detect glycoprotein B (gB) gene of pseudorabies virus. Pseudorabies virus (PRV)-gB gene amplified by PCR was cloned into pMD20-T vector and it was used as positive standard. The assay thus developed, can be used for early easy detection of PRV,and for quarantine of import and export of meat products.
Databáze: Supplemental Index