| Autor: |
Chen, Li, Li, Bin, Yang, Wu–Chen, He, Jia–Lin, Li, Ning–Yi, Hu, Jian, He, Ya–Fei, Yu, Shu, Zhao, Zhuo, Luo, Ping, Zhang, Jin–Yong, Li, Hai–Bo, Zeng, Ming, Lu, Dong–Shui, Li, Bo–Sheng, Guo, Hong, Yang, Shi–Ming, Guo, Gang, Mao, Xu–Hu, Chen, Weisan |
| Předmět: |
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| Zdroj: |
Gastroenterology (00165085); Mar2013, Vol. 144 Issue 3, p591-600, 10p |
| Abstrakt: |
Background & Aims: Immunodominance is an important feature of antiviral, antitumor, and antibacterial cellular immune responses, but it is not well demonstrated in the immune responses against Helicobacter pylori. Antigen-specific CD4+ T cells protect mice against infection with H pylori. We investigated the immunodominant CD4+ T-cell response to neuraminyllactose-binding hemagglutinin (HpaA), which is a conserved, H pylori–specific colonization factor that is being investigated as an antigen for vaccination strategies. Methods: HpaA-specific CD4+ T cells were expanded with autologous peripheral blood mononuclear cells that had been incubated with recombinant HpaA and characterized using overlapping synthetic peptides. We compared the percentage of CD4+ T cells with specificity for HpaA88–100, restricted to HLA-DRB1*1501, among 59 H pylori–infected subjects with different gastric diseases. Results: We identified and characterized several immunodominant CD4+ T-cell epitopes derived from HpaA. The immunodominant CD4+ T-cell responses specific to HpaA88–100 were observed in most H pylori–infected individuals who expressed HLA-DRB1*1501 and were significantly more abundant in patients with less severe diseases (P < .05). Conclusions: The HLA-DRB1*1501–restricted immunodominant CD4+ T-cell response to HpaA88–100 is associated with reduced risk of severe gastric diseases. Further study of these and other immunodominant CD4+ T-cell responses to H pylori will provide insight into mechanisms of protective immunity and aid in vaccine design. [ABSTRACT FROM AUTHOR] |
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