| Autor: |
Omori, Katsunori, Wada, Shigehito, Maruyama, Yusuke, Hattori, Atsuhiko, Kitamura, Kei-Ichiro, Sato, Yusuke, Nara, Masayuki, Funahashi, Hisayuki, Yachiguchi, Koji, Hayakawa, Kazuichi, Endo, Masato, Kusakari, Rei, Yano, Sachiko, K. Srivastav, Ajai, Kusui, Takashi, Ejiri, Sadakazu, Chen, Wenxi, Tabuchi, Yoshiaki, Furusawa, Yukihiro, Kondo, Takashi |
| Zdroj: |
Zoological Science (Zoological Society of Japan); Aug2012, Vol. 29 Issue 8, p499-504, 6p |
| Abstrakt: |
Using our original in vitro assay system with goldfish scales, we examined the direct effect of prostaglandin E2 (PGE2) on osteoclasts and osteoblasts in teleosts. In this assay system, we measured the activity of alkaline phosphatase (ALP) and tartrate-resistant acid phosphatase (TRAP) as respective indicators of each activity in osteoblasts and osteoclasts. ALP activity in scales significantly increased following treatment at high concentration of PGE2 (10-7 and 10-6 M) over 6 hrs of incubation. At 18 hrs of incubation, ALP activity also significantly increased in the PGE2 (10-7 to 10-6 M)-treated scale. In the case of osteoclasts, TRAP activity, tended to increase at 6 hrs of incubation, and then significantly increased at 18 hrs of incubation by PGE2 (10-9 to 10-6 M) treatment. At 18 hrs of incubation, the mRNA expression of osteoclastic markers (TRAP and cathepsin K) and receptor activator of the NF-KB ligand (RANKL), an activating factor of osteoclasts expressed in osteoblasts, increased in PGE2 treated-scales. Thus, PGE2 acts on osteoblasts, and then increases the osteoclastic activity in the scales of goldfish as it does in the bone of mammals. In an in vivo experiment, plasma calcium levels and scale TRAP and ALP activities in the PGE2-injencted goldfish increased significantly. We conclude that, in teleosts, PGE2 activates both osteoblasts and osteoclasts and participates in calcium metabolism. [ABSTRACT FROM AUTHOR] |
| Databáze: |
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