| Autor: |
Mavraki, E., Moschou, D., Kokkoris, G., Vourdas, N., Chatzandroulis, S., Tserepi, A. |
| Zdroj: |
Procedia Engineering; Apr2012, Vol. 25, p1245-1248, 4p |
| Abstrakt: |
Abstract: The purpose of this work is to develop a simple and low cost microfluidic device with integrated microheaters on a thin flexible polymeric substrate, in order to perform DNA amplification on chip, at faster amplification rates due to the reduced thermal capacity and large heat transfer rate between the sample and the temperature-controlled heating zones. We fabricate a continuous-flow PCR microfluidic device, developed on a commercially available substrate (PyraluxTM) and we focus on the electrical and thermal characterization of the integrated microheaters. Each microheater defines one of the three temperature zones through which the DNA sample will be cycled for 25 times as it flows continuously through the microchannel. Electrical measurements confirm the functionality of the microheaters both as temperature sensors and thermal elements. Heat transfer and fluid dynamics computations are also performed to ascertain that the temperature of each microheater is almost identical to that in the microchannel crossing the corresponding thermal zone. [Copyright &y& Elsevier] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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