| Abstrakt: |
The variety of grape-wine Feteasca Neagra was tested on mediums of culture with small doses of cytokinine, in comparison with doses of five or ten times greater. The apical and nodal tissue of about 1,5-2,0 mm separated from shoots forced in growth room has been inoculated in early spring (March), on the fallowing options: Vo=MS; V1=MS1/2 +3g/l charcoal; V2=MS +1,0mg/l BA + 0,5 mg/l AIB; V3=MS+5,0mg/l BA+0,5mg/l AIB; V4=MS +10mg/l BA+0,5 mg/l AIB. Regeneration rate was monitored (after 20, 40, 60, 80 days), and also the evolution of the tissue after 80 days, with reference to shoot induction, the formation of the root system and the appearance of some atypical formations. After two months of culture in vitro we can say that the favorable variant is the one without growth hormones and with a plus of charcoal (Vo and V1), on which a smaller number of neoplantules, fully organized, have appeared, which are high and with a well-developed root system. On the variant with 10 mg/l BA have appeared about 2-3 very small plantules, of 0,5cm embedded in a mass of rhizogenic calus, unprecedented phenomenon, apparently caused by the high dose of cytokinine. The 5mg/l BA concentration from the medium generates the greater number of plants, about 12 from an explant of 2 cm with an appropriate number of roots and a calus sleeve of about 2 mm around the neoplantules. [ABSTRACT FROM AUTHOR] |
