| Abstrakt: |
In 3 groups, each of 15 broiler chickens ochratoxine A (OTA) was given orally, in sunflower oil suspension, daily, for 21 days in doses of: 5μg/kg b.w. for group E1, 35 μg/kg for group E2 and 100 μg/kg for E3. Control group (of 15 chickens) received only sunflower oil. 5 chickens from each group were killed after 7, 14 and 21st day of the experiment. Skin samples were taken from the head, chest, wings and coccigian region and gut mucosa samples were prevealed from duodenum, jejunum and coecal tonsils. All samples were prepared for paraphin embeding and stained with: HEA, PAS, PAS and Alcian blue and May Grunwald Giemsa and lymphoid associated tissue was examined. Skin lesions were observed in E2 (receiving 5μgOTA/kg b.w./day) and E3 group (receiving 35μg OTA/kg b.w./day) at 7th day of poisoning, consisting in dermis oedema, ectasia of the capillaries (filled with erytrocytes) and extravasation of blood cells. In the 14th day of poisoning denudation of epidermis, degenerated, plumiferous follicles, dermic haemorrhages and many oriented conjonctive fibers were observed. Few lymph cells were observed in the dermis. In duodenum, the cryptae from lamina propria showed numerous immature young cells, with nucleus rich in eucromatine, with 3-4 nucleoli. The enterocytes had a large nucleus rich in euchromatine, with 3-4 nucleoli. Many goblet cells were observed into duodenal mucosa. Progressively with the age and OTA dosis the lipidic droplets from the axis of the villi and proportion of degenerated enterocytes are increasing and simple epithelium is replaced by stratified epithelium, with many immature cells. The coecal tonsils in E2 group, after 14 days of OTA poisoning showed lamina propria populated by small lymph cells. Some of the lymph cells show nuclear lesions like: cariorhexis, cariolysis and other show apoptotic aspects. Into the lymphoid agglomerations some cells with nuclei rich in eucromatina, with 4-5 nucleoli were observed. [ABSTRACT FROM AUTHOR] |
