| Abstrakt: |
Separate transcriptomes were constructed by pyrosequencing on the GS-FLX sequencer (Roche), for the whole body and digestive system of the adult Tarnished plant bug, Lygus lineolaris, collected from the field in North Carolina, USA. Each cDNA library from the two samples examined were sequence on one-half plates each. The total ouput from the two reads was 116, 635, 527 bps of data. The whole body library produced 292,714 reads which were assembled using the GS Assembler ver. 1.1.02.15 (Roche) with default parameters into 5,529 contiguous sequences (contigs). The digestive system library produced 229,919 reads which was assembled into 3,549 contigs. Contigs were putatively identified using the Tera-BLASTX algorithm with DeCypher (TimeLogic) against Genbank nr and est databases that were downloaded on June 2008. The contigs obtained from the tarnished plant bug gut transcriptome were analyzed using the blast2go program (www.blast2go.org). Blast2go also blasted the contigs using its blastx function and performed the gene ontologies. The analysis of the results from the whole bug and gut transcroptomes are in its infancy. However, the gut library has some obvious messages that should be of interest to plant, genetic pest management. [ABSTRACT FROM AUTHOR] |
