| Abstrakt: |
AIM: To assess the effect of BMP4 morphogenetic factor on alveolar osteogenesis and odontoblast differentiation, aiming at future clinical implementation of results. STUDY DESIGN:Static Group. TIME AND PLACE OF STUDY:This study is part of the doctoral thesis of the first author at the Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, Athens, Greece. Research involves injection of anti-BMP4 in pregnant mice and study of effects in newborn subjects. MATERIAL AND METHODS: Each experimental group comprised 20 subjects. The experimental group of pregnant white mice (Mus musculus), was subjected to the effect of monoclonal antibody anti-BMP4 during the 9th gestational day. Results were evaluated, (a) macroscopically, (b) stereoscopically, following double histochemical staining of cartilage/osseous tissue, and with photon microscopy following (c) histological staining (Domagk) of paraffin sections, and (d) immunohistochemistry. RESULTS: In vivo administration of a certain quantity of monoclonal anti-BMP4 antibody does not seem to affect the general development of the mature organism. However, at the dental level, there is transitory interference with the normal course of odontoblast differentiation and the production of pre-dentin, whereas delay in ossification also includes the alveoli. CONCLUSIONS: Controlled, experimental anti-BMP4 administration seems to be both safe for the organism and promising for intended, guided odontoblast differentiation. [ABSTRACT FROM AUTHOR] |
