Autor: |
Dong, H., Fan, Y.-H., Wang, Y.-Y., Wang, W.-T., Hu, S.-J. |
Předmět: |
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Zdroj: |
Physiological Research; 2008, Vol. 57 Issue 4, p639-645, 7p, 3 Graphs |
Abstrakt: |
The aim of this study was to determine the effect and mechanism of low concentration of lidocaine on subthreshold membrane potential oscillations (SMPO) and burst discharges in chronically compressed dorsal root ganglion (DRG) neurons. DRG neurons were isolated by enzymatic dissociation method. SMPO, burst discharges and single spike were elicited by whole cell patch-clamp technique in current clamp mode. Persistent Na+ current (INap) and transient Na+ current (INaT) were elicited in voltage clamp mode. The results showed that SMPO was suppressed and burst discharges were eliminated by tetrodotoxin (TTX, 0.2 μmol/l) in current clamp mode, INap was blocked by 0.2 μmol/l TTX in voltage clamp mode. SMPO, burst discharges and INaP were also suppressed by low concentration of lidocaine (10 μmol/l) respectively. However, single spike and INaT could only be blocked by high concentration of lidocaine (5 mmol/l). From these results, it is suggested that INaP mediates the generation of SMPO in injured DRG neurons. Low concentration of lidocaine (10 μmol/l) suppresses SMPO by selectively inhibiting INaP, but not INaT, in chronically compressed DRG neurons. [ABSTRACT FROM AUTHOR] |
Databáze: |
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