| Autor: |
McMillan, James D., Adney, William S., Mielenz, Jonathan R., Klasson, K. Thomas, Ishida, Nobuhiro, Saitoh, Satoshi, Ohnishi, Toru, Tokuhiro, Kenro, Nagamori, Eiji, Kitamoto, Katsuhiko, Takahashi, Haruo |
| Zdroj: |
Twenty-Seventh Symposium on Biotechnology for Fuels & Chemicals; 2006, p795-807, 13p |
| Abstrakt: |
We developed a metabolically engineered Saccharomyces cerevisiae, which produces optically pure L-lactic acid efficiently using cane juice-based medium. In this recombinant, the coding region of pyruvate decarboxylase (PDC)1 was completely deleted, and six copies of the bovine L-lactate dehydrogenase (L-LDH) genes were introduced on the genome under the control of the PDC1 promoter. To confirm optically pure lactate production in low-cost medium, cane juice-based medium was used in fermentation with neutralizing conditions. L-lactate production reached 122 g/L, with 61% of sugar being transformed into L-lactate finally. The optical purity of this L-lactate, that affects the physical characteristics of poly-L-lactic acid, was extremely high, 99.9% or over. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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