| Autor: |
Bernard, A., Griffiths, B., Noé, W., Wurm, F., Hendrick, V., Vandeputte, O., Raschella, A., Marique, T., Cherlet, M., Abdelkafi, C., Werenne, J. |
| Zdroj: |
Animal Cell Technology: Products from Cells, Cells as Products; 2000, p179-181, 3p |
| Abstrakt: |
Efficient t-PA production in recombinant CHO cells is of major interest for pharmaceutical industry. Contrary to the multigene metabolic engineering approach, our strategy allows investigations of recombinant cell lines already validated. Compared to 37°C, 32°C showed that t-PA productivity was significantly increased. The specific rate of t-PA secretion was enhanced at the lower temperature, in relation to the cell cycle modification. At this temperature, glycosylation is not significantly altered while serine protease activity is reduced. A similar study involving cytofluorimetric data and mathematical analysis was made for the other factors tested (PMA, TGF-β and butyrate). Our data not only emphasize the interest of a two step process for t-PA production (involving 1. a cell biomass production phase 2. a high protein productivity phase), but showed moreover that productivity can be furthermodulated by the extracellular environmental factors affecting cell cycle. On the basis of results obtained by our rapid screening method a multigen engineering strategy could be decided on a rational basis. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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