| Autor: |
Walker, John M., Scanlon, Kevin J., Yuan, Yan |
| Zdroj: |
Therapeutic Applications of Ribozymes; 1998, p415-425, 11p |
| Abstrakt: |
RNase P is an enzyme that cleaves tRNA precursors to generate the 5′ termini of mature tRNAs (1). Recently, the author developed a method for specific inactivation of any RNA using human RNase P (2). This method involves the design of a small RNA, known as external guide sequence (EGS). EGS RNA, when complexed with the target RNA, generates a structure that resembles a model substrate derived from a tRNA precursor, thereby rendering the target RNA susceptible to cleavage by RNase P. The methodology and application of the EGS strategy is described in Chapter 26. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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