| Autor: |
Phillips, Ian R., Shephard, Elizabeth A., Vinken, Mathieu, Elaut, Greetje, Henkens, Tom, Papeleu, Peggy, Snykers, Sarah, Vanhaecke, Tamara, Rogiers, Vera |
| Zdroj: |
Cytochrome P450 Protocols (9781588294418); 2006, p247-254, 8p |
| Abstrakt: |
Mimicking the in vivo microenvironment is one of the current strategies to maintain liver-specific functionality in primary cultured hepatocytes for long periods. Freshly isolated hepatocytes entrapped in collagen gel type I (collagen gel immobilization culture) or sandwiched between two layers of hydrated collagen type I (collagen gel sandwich culture) are known to display liver-specific functions (e.g., biotransformation capacity) for more than 6 wk. We describe how to set up both types of organotypical hepatocyte culture systems. Besides a detailed protocol, we give some practical tips, taken from our own experience with long-term hepatocyte culture. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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