| Autor: |
Walker, John M., Liu, X. Johné, Morris, Catherine E., Juranka, Peter F., Wei Lin, Morris, Terence J., Laitko, Ulrike |
| Zdroj: |
Xenopus Protocols; 2006, p315-329, 15p |
| Abstrakt: |
The mechanosensitivity of voltage-gated (VG) channels is of biophysical, physiological, and pathophysiological interest. Xenopus oocytes offer a critical advantage for investigating the electrophysiology of recombinant VG channels subjected to membrane stretch, namely, the ability to monitor macroscopic current from membrane patches. High-density channel expression in oocytes makes for macroscopic current in conventional-size, mechanically sturdy patches. With the patch configuration, precisely the same membrane that is voltage-clamped is the membrane subjected to on-off stretch stimuli. With patches, meaningful stretch dose responses are possible. Experimental design should facilitate within-patch comparisons wherever possible. The mechanoresponses of some VG channels depend critically on patch history. Methods for minimizing and coping with interference from endogenous voltage-dependent and stretch-activated endogenous channels are described. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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