An In Vitro Transcription System for Studying Vaccinia Virus Late Genes.

Autor: Walker, John M., Isaacs, Stuart N., Wright, Cynthia F.
Zdroj: Vaccinia Virus & Poxvirology; 2004, p143-150, 8p
Abstrakt: This chapter describes a protocol that allows accurate in vitro transcription of vaccinia virus late genes. In this method, extracts are made from vaccinia virus-infected cells and used as enzyme sources to produce mRNAs from plasmid templates containing late gene promoter sequences. [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index