| Autor: |
Walker, John M., Tymms, Martin J., Watt, Fujiko |
| Zdroj: |
Transcription Factor Protocols; 2000, p257-264, 8p |
| Abstrakt: |
In vitro transcription provides information not necessarily available from transfection assays about the regulatory activity of a promoter transcribed by RNA polymerase II. In the particular application discussed here, when an oligonucleotide duplex containing a recognition site for a specific transcription factor is included in the transcription reaction using nuclear extract, the factor can be selectively depleted from a pool of binding factors that are required to regulate transcription from the class II promoter. [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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