A computer program based on the psi function for model-independent analysis of sedimentation equilibrium distributions reflecting macromolecular interactions.

Autor: Kremer, F., Lagaly, G., Jaenicke, R., Durchschlag, H., Cölfen, H., Winzor, D. J.
Zdroj: Analytical Ultracentrifugation IV; 1997, p36-42, 7p
Abstrakt: A computer program, written in BASIC, has been developed for the characterization of either solute self-association or the interaction between macromolecular acceptor and ligand. Simplicity and model independence of the procedure to evaluate the free concentration of smallest macromolecular species are features which are illustrated by analysis of simulated Rayleigh distributions based on α-chymotrypsin dimerization, and of simulated absorbance records of the distribution based on 1:1 interaction between trimethylamine dehydrogenase (acceptor) and electron transferring flavoprotein (ligand). [ABSTRACT FROM AUTHOR]
Databáze: Supplemental Index