| Autor: |
Walker, John M., Smales, C. Mark, James, David C., Horenstein, Alberto L., Durelli, Ilaria, Malavasi, Fabio |
| Zdroj: |
Therapeutic Proteins; 2005, p191-208, 18p |
| Abstrakt: |
Monoclonal antibodies (MAbs)—reagents mass-produced in the laboratory to recognize a specific molecular target (1)—are currently the most widely used proteinbased therapeutic and diagnostic molecules in clinical trials (2). This chapter presents the most effective ready-to-use protocol for MAb purification of clinical-grade standard. Samples of high purity are often produced via a highly selective single step. However, an appropriate combination of chromatographic materials in at least two orthogonal (i.e., based on two different mechanisms) steps is mandatory for obtaining clinical-grade MAbs for the in vitro functional analysis of ligand signals on cell-based assays and protein expression changes on arrayed MAb biochips (3), or in vivo preclinical and therapeutic applications (4). [ABSTRACT FROM AUTHOR] |
| Databáze: |
Supplemental Index |
| Externí odkaz: |
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