| Abstrakt: |
Reversed-phase HPLC (RP-HPLC) is now well established as a technique for isolation, analysis, and structural elucidation of peptides and proteins (1,2). Its use in protein isolation and purification may have reached a peak owing to recent developments in high efficiency ionexchange and hydrophobic interaction supports, which are now capable of equivalent levels of resolution to RP-HPLC without concomitant risk of denaturation and loss of biological activity. Nevertheless, there are many applications in which denaturation may be unimportant and high concentrations of organic modifier can be tolerated (e.g., purity analyses, structural studies, and micropreparative purification prior to microsequencing). It is a widely used tool in Biotechnology for process monitoring, purity studies, and stability determinations. [ABSTRACT FROM AUTHOR] |
